---CRP kit literature pdf---
{{ :nchsls:c:biochemistry:document:crp_kit_lit.pdf |}}
---CRP Calibration with linearity establishment---
{{ :nchsls:c:biochemistry:document:crp_plan.ods |}}
     * lyophilized crp bottle is reconstituted with 1 ml DI water, incubated for 10 to 15 minutes with periodic shaking until solutes becomes completely dissolved.
     * 100 Ul of the mixture is added with 100 Ul of Di water. 
     * from there again 100 Ul of solution is added to the next alliquote and 100 Ul water is added for 3 more subsequent times. (serial dilution)     
     * For establishing linearity a total of 6 samples are required.
     * 1 sample is the plain calibrator(as CRP stock), and the other one is DI water(as blank).
     * the samples are analysed in the analyser and od is plotted in the graph.
     * from there the factors are extracted and feeded in the machine.
     * the R1 volume is change from 200 UL to 160 ul and r2 volume is changed from 50 to 40 ul. 
     * special wash reagent (0.1 n NAOH) is also added for wash

======ERBA XL 1000======
|[[Centrifugation,Preparing aliquot,how_to_perform_examination_in_XL-1000]]| 


Calibrator traceability cerficate {{ :nchsls:c:biochemistry:document:tc_c062101.pdf |}}