======Creatinine examination procedure======
  *Learn operation of [[Erba XL-640]] and [[Erbachem]].
  *Select equipment from the list below.
    *Use Erba XL-640 as peferred equipment.
    *If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow [[procedure for responding to Erba XL-640 failure]]
  *Read following documents
 
^references|{{creatinine_reference_literature_1.pdf|creatinine_reference_literature_1}}|
| |{{creatinine_reference_literature_2.pdf|creatinine_reference_literature_2}}|
| |{{creatinine_reference_literature_3.pdf|creatinine_reference_literature_3}}|
| |{{creatinine_reference_literature_4.pdf|creatinine_reference_literature_For R1_1}}|
| |{{creatinine_reference_literature_5.pdf|creatinine_reference_literature_For R1_2}}|
| |{{creatinine_reference_literature_6.pdf|creatinine_reference_literature_For R1 with SDS}}|
| |**Note:** When a new lot is opened, verify kit literature with the uploaded version.|
| |[[lis_upload_dokuwiki_media|Upload]] newer version when required and make necessary changes in procedure and process| 

|**environmental and safety controls**|{{msds_NAOH.pdf|MSDS of NAOH}}|
| |{{msds_BRIJ35%.pdf|MSDS of BRIJ35%}}|
| |{{msds picricacid.pdf|MSDS of picric acid}}|
| |{{msds of SDS.pdf|MSDS of SDS}}|

|**Preparation of [[Creatinine reagent]]**| 

[[nchsls:c:biochemistry:document:online_information_resource_for_patients_and_users#Instructions_for_Patient_Collected_Samples|Special instructions for patients]]

|The examination is done on Erba XL-640. It is also done on Erbachem when Erba XL-640 is damaged and repair may take longer|

======Erba XL-640======

|[[Centrifugation]]|
|[[Preparing aliquot]]|
|[[nchsls:c:biochemistry:document:how_to_perform_examination_in_erba_xl-640|Procedure for examination in Erba XL-640]]|
======Erbachem======
**Note:** Following instruction is useful only if operator is familiar with [[Erbachem]]

^Parameter^Value^
^Sample volume(ul)|50|
^Reagent volume(ul)|500|
^Filter|505 nm|
^Aspitation Volume(ul)|300|
^Type of reaction|RATE-A|
^Decimal place|1|
^Linearity|1-20mg/dl|
^Delay time |30 sec|
^Reading time |60 sec|

^Calibration, Result calculation, Quality control and sample examination in Erbachem^

^[[nchsls:c:biochemistry:document:internal_quality_control|Internal Quality Control]]^
^[[how_to_do_external_quality_control_on_erba_xl-640|EQA]]^

^Prepare for calibration as below every monthly or there is relevent NC. Refer [[Calibrator and QC]]^
^testing material^Replicates^
|5% Albumin blank|4|
|Normal Range Calibrator|4|
|Abnormal range Calibrator|4|
|Normal range QC sera|4|
|Abnormal Range QC sera|4|

  -Run all testing material as sample (as described below at **Sample Examination**)
  -Evaluate calibration and QC absorbances in spreadsheet. Compare with past results.
  -If results are acceptable, enter the factor in the erbachem and set decimal place to zero.

====sample examination====
  -Check aspiration volume of 300 microliter in test parameter of Creatinine.
  -Run Randox Human assy control-2(QC 5),1 Randox Human assy control-3(QC 8) in each and every batch. See Assigned values of target values of QC [[Calibrator and QC Targets|here]]
  -Select "Run test" -> CRE in Erbachem menu.
  -When instrument asks "Aspirate water", aspirate water.
  -When instrument asks "Aspirate sample".
  -Mix Creatinine reagent R1 and R2 in equal quantity.
  -Take 500 microliter of mix (R1+R2) reagent in ependroff cup 			
  -add 50 microliter sample to it  	
  -Take reading immediately at 505 nm.	
  -Total time taken for analysis is 90 seconds. (delay time 30 seconds + reading time 60 seconds)
  -Evaluate QC results. If QC results show major deviation from past, rerun batch.
  -If result is more than 20.0mg/dl then dilute the sample with DI water and rerun the sample.
  -[[lis_calibration_entry|Enter Calibration in LIS]]
  -[[list_qc_entry_manual|Enter QC results manually in LIS]]
  -[[list_result_entry_manual|Enter sample results manually in LIS]]
  -**instructions for determining quantitative results when a result is not within the measurement interval:** Dilution of sample with DI water is done in same way as done by Erba XL-640  automatically.
 

  - Currently serum and urine are accepted as sample type.