====How to prepare 1% Agarose gel====
  -Dissolve 150 mg of pure agarose in 15 ml of TEB buffer (pH 8.6).  
  -Boil it to dissolve it completely &  make  a homogeneous &  clear  solution. 
  -To make sure that agarose powder dissolve completely,look under the glassware in light.Be careful for your eyes.