====1% Agarose Gel Preparation====
  -Dissolve 100 mg of pure agarose in 10 ml of [[ glycine-Tris buffer ( Half Diluted) ]] pH 8.6.
  -Weight entire flask along with agarose and buffer. Note it down.
  -Boil it to dissolve it completely &  make  a homogeneous &  clear  solution.
  -Weight the boiled solution and add DI water equivalent to evaporated volume.  
  -Again boiled the solution to mixed it properly.  
  -To make sure that agarose powder dissolve completely,look under the glassware in light.Be careful for your eyes.