====How to prepare 1% Agarose gel====
  -Dissolve 100 mg of pure agarose in 10 ml of hippurate buffer pH 8.6.
  -Boil it to dissolve it completely &  make  a homogeneous &  clear  solution.
  -To make sure that agarose powder dissolve completely,look under the glassware in light.Be careful for your eyes.