Dr Piyush Tailor

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===PROCEDURE FOR WASHING CUPS FOR QC AND CALIBRATORS===
 -Wear goggles and gloves. 
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 -Take all the new cups in a rack. 
{{ :nchsls:c:biochemistry:document:img_20161129_110117.jpg?nolink&400 |}}
 -Pour 10% HCL in squeezing bottle
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 -Pour 10% HCL in each cup at a time
{{ :nchsls:c:biochemistry:document:img_20161129_111246.jpg?nolink&400 |}} 
 -Wait for 10-15mins.
 
 -Wash all the cups individually with de-ionised water.
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 -Pour methanol in squeezing bottle
{{ :nchsls:c:biochemistry:document:img_20161129_105947.jpg?nolink&400 |}}
 -Pour methanol in each cup at a time
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 -**DO NOT DISCARD METHANOL**.Collect it in Resin carbo.Reuse for electrophoresis.

-Wash each and every cup with deionised water. Repeat it for 4 times.
{{ :nchsls:c:biochemistry:document:img_20161129_113438.jpg?nolink&400 |}}
 -Get rid of all the water in cups and keep them in a tray.Tray should be covered with Gauze piece.
{{ :nchsls:c:biochemistry:document:img_20161129_113453.jpg?nolink&400 |}}
 -Keep the tray in an incubator until dry at temp of 37'C(**DO NOT USE MORE THAN 37'**Because high temp change the shape of the cups).

-Now the cups are ready to use. Currently QC Cups not washed before use since 2nd October 2018.

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Dr Piyush Tailor

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