Dr Piyush Tailor

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====How to prepare HbA ,HBS and HBF control====
===For HBF Control preparation===
   -Collect about 2-4 ml cord blood in plastic bottle containing 2 ml CPDA
     -Alternatively add __washed RBC of new born EDTA sample__ in 2 ml CPDA bottle till total volume become 4 ml approximately. Preserve at 2-8'C till required amount is collected.
     -Use washed RBC to prevent mismatch agglutination in pooled RBC
   -Wash with saline twice in 4 ml EDTA vacumm tube. Remove Saline part
   -After washing,Add Preservative CPDA in washed RBCs in 1:1 ratio.
   -Prepare aliquots of 50 ul.
   -Label and store at 2-8'C.

===For HBA+HbS Control preparation===
     -Add __washed RBC of HbA or HbS or HbA+HBS EDTA sample__ in 2 ml CPDA bottle till total volume become 4 ml approximately.
     -Use washed RBC to prevent mismatch agglutination in pooled RBC
   -Wash with saline twice in 4 ml EDTA vacumm tube. Remove Saline part
   -After washing,Add Preservative CPDA in washed RBCs in 1:1 ratio. 
   -Prepare aliquots of 50 ul.
   -Label and store at 2-8'C.

===For HBA+HbS+HbF Control preparation===
  -Mixed HBA+HbS and HbF control in equal amount from above.
   -Prepare aliquots of 50 ul.
   -Label and store at 2-8'C.

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Dr Piyush Tailor

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