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md_biochemistry [2024/11/10 11:53] admin created |
md_biochemistry [2025/08/20 09:15] (current) admin |
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| - Write type of transport mechanism and explain with example | - Write type of transport mechanism and explain with example | ||
| + | - Clinical impact of the different transport mechanism. Explain with two example of either disease or drug) | ||
| + | - Lysozyme and it’s significance with medical diseases. | ||
| + | - Principle, type and use of CLIA | ||
| + | - Application of zwitter ion and protein’s primary structure in pharmaco-kinetic during therapeutic use of different type of insulin. - Explain with example. | ||
| - Biochemical events occurring during skeletal muscle contraction. | - Biochemical events occurring during skeletal muscle contraction. | ||
| - Biochemical difference between muscle fibers and fuel used by sprinter and marathon runner | - Biochemical difference between muscle fibers and fuel used by sprinter and marathon runner | ||
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| - EPA and DHA – biochemical basis of their role in health | - EPA and DHA – biochemical basis of their role in health | ||
| - Chaperones | - Chaperones | ||
| + | - Explain principle, advantages and disadvantages of quantitative estimation of glucose through Ion Selective Electrode, GOD-POD method, Benedict method, urine strip method and with Glucometer. | ||
| - Source and clinical significant of essential fatty acid | - Source and clinical significant of essential fatty acid | ||
| - Collagen synthesis and its disorder | - Collagen synthesis and its disorder | ||
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| - Type of Chromatography | - Type of Chromatography | ||
| - Principle and instrumentation of nephelometry and turbidimetry | - Principle and instrumentation of nephelometry and turbidimetry | ||
| - | - Principle | + | - Type and factor affecting electrophoresis. Write principle , component , significant of the each component |
| - Principle and utility of mass spectrometry | - Principle and utility of mass spectrometry | ||
| - Principle, type and use of ELISA. | - Principle, type and use of ELISA. | ||
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| - Electrophoresis support media | - Electrophoresis support media | ||
| - Isoelectric focusing | - Isoelectric focusing | ||
| - | - Principle of SDS PAGE | ||
| - Principle, instrumentation and uses of capillary electrophoresis | - Principle, instrumentation and uses of capillary electrophoresis | ||
| - Affinity chromatography | - Affinity chromatography | ||
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| - Amperometric O2 based and H2O2 based glucose electrodes | - Amperometric O2 based and H2O2 based glucose electrodes | ||
| - Mechanism of methotrexate and 5-flurouracil | - Mechanism of methotrexate and 5-flurouracil | ||
| + | - Technical requirement to maintain integrity of the sample between sample collection to analysis as per clause 7.2.5 and 7.2.7.3 of ISO 15189:2022 and expected risk on sample stability for biochemistry analytes. | ||
| + | - Write two enzyme with it’s therapeutic use with it’s mechanism of action. | ||
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| - Explain structure , type, variant , physiological role, synthesis and regulation of hemoglobin | - Explain structure , type, variant , physiological role, synthesis and regulation of hemoglobin | ||
| - Digestion and absorption of protein | - Digestion and absorption of protein | ||
| + | - Organo-phosphorus poisoning - Biochemical explanation behind the it’s effect , clinical feature, diagnosis and management. | ||
| + | - Pathway, significance and regulation of gluconeogenesis | ||
| + | - Define chaperon. Explain pathogenesis of prion disease with example of amyloidosis. | ||
| - Dietary fiber – It’s significant and biochemical reason | - Dietary fiber – It’s significant and biochemical reason | ||
| - Regulation of enzyme activity. | - Regulation of enzyme activity. | ||
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| - Potentiometry electrodes for pCO2 | - Potentiometry electrodes for pCO2 | ||
| - Amperometric electrode for pO2 | - Amperometric electrode for pO2 | ||
| + | - Defined classification of laboratory as per NABL-112 criteria. | ||
| + | - Enumerate non-human resources for the biochemistry section as per large size laboratory. Write specification for any one major resource (instrument / reagent / consumable) of each area of the laboratory (total 3) . | ||
| + | - Write performance validation protocol for these specified resource ( instrument / reagent / consumable) | ||
| + | - Write a consistent quality policy and objective with defined quality indicator, for your laboratory. | ||
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