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definitions [2025/02/24 10:36] admin |
definitions [2025/02/24 10:48] (current) admin |
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| + | ** Factor of The Analyte =** Concentration of Calibrator / (O.D. of Calibrator - O.D. of Blank ) | ||
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| **Concentration of Substrate / Analyte =** (O.D. of Substrate - O.D. of Blank ) x Concentration of Calibrator / (O.D. of Calibrator - O.D. of Blank ) | **Concentration of Substrate / Analyte =** (O.D. of Substrate - O.D. of Blank ) x Concentration of Calibrator / (O.D. of Calibrator - O.D. of Blank ) | ||
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| + | **Concentration of Substrate / Analyte =** (O.D. of Substrate - O.D. of Blank ) x Factor | ||
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| + | **Delta OD =** (OD at 60 second) - (OD at 0 Second) = Difference of OD in 60 Second (1 minute) interval | ||
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| + | Note : In case of kinetic or End-Point kinetic method, Delta OD will be used instead of OD | ||
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